A 1–6 GHz analog radio frequency power driver in 90 nm complementary metal‐oxide semiconductor technology for wireless applications

One of the most challenging subsystems for integrated radio frequency (RF) complementary metal‐oxide semiconductor (CMOS) solutions is the power amplifier. A 1–6 GHz RF power driver (RFPD) in 90 nm CMOS technology is presented, which receives signals from on‐chip RF signal chain components at ?12 dBm power levels and produces a 0 dBm signal to on‐chip or off‐chip 50 Ω loads. A unique unit cell design is developed for the RFPD to offset issues associated with very wide multi‐fingered transistors. The RF driver was fabricated as a stand‐alone sub‐circuit on a 90 nm CMOS die with other sub‐circuits. Experimental tests confirmed that the on‐chip RFPD operates up to 6 GHz and is able to drive 50 Ω loads to the desired 0 dBm power level. Spur free dynamic range exceeded 70 dB. The measured power gain was 11.6 dB at 3 GHz. The measured 1 dB compression point and input third‐order intercept point (IIP3) were ?4.7 dBm and ?0.5 dBm, respectively. Also, included are modeling, simulation, and measured results addressing issues associated with interfacing the die to a package with pinouts and the package to a printed circuit test fixture. The simulations were made through direct current (DC), alternating current (AC), and transient analysis with Cadence Analog Design Environment. The stability was also verified on the basis of phase margin simulations from extracted circuit net‐lists. Copyright © 2013 John Wiley & Sons, Ltd.     

Acid phosphatase in the tubers of Dioscorea species and its purification from the white yam (D. rotundata poir)

Acid phosphatase activity was determined in 15 cultivars from four species of yam. A 12-fold purification of the enzyme from Dioscorea rotundata (cv. chikakwondo) gave a homogeneous preparation as demonstrated by polyacrylamide gel electrophoresis. This enzyme preparation has an apparent molecular weight of 115 000 ±2000 and an optimum activity at a pH of 5·20 and a temperature of 50°C. The K_m of the enzyme is 3·81 mM with disodium p-nitrophenylphosphate (p-NNP) as a substrate. The energy of activation, heat of activation, energy of inactivation and heat of inactivation are 7·0, 6·4, 4·41 and 4·34 kcal M^?1, respectively. Although it has very little activity with most organic phosphoric acid esters, it is significantly inhibited by Ca²⁺, Hg²⁺ and EDTA and activated by Mg²⁺. The enzyme has a half-life of 50,17 or 13 days, respectively, when stored at 6-8°C, 0°C or room temperature (29±2°C).     

Electromagnetic Torque Estimation of a Three-Phase Induction Motor for Setting the Feedrate of a Milling Machine

In this paper, it presents a project of a fuzzy controller and a neural estimator to control a coordinate table powered by three-phase induction motor, aiming to implement an intelligent milling system. The position/speed control is performed using vector techniques of three-phase induction machines. The estimation of the motor electromagnetic torque is used for setting the feedrate of the table. The speed control is developed using TS （Takagi-Sugeno） fuzzy logic model and electromagnetic torque estimation using neural network type LMS （least mean square） algorithm. The induction motor is powered by a frequency inverter driven by a DSP （digital signal processor）. Control strategies are implemented in DSP. Simulation results are presented for evaluating the performance of the system.     

Influence of recovery conditions on apparent heat resistance of Bacillus stearothermophilus spores

The effects of post-treatment environmental factors on the heat resistance of Bacillus stearothermophilus spores (ATCC 12980, 7953, 15951 and 15952) were investigated. Nutrient Agar (NA), Antibiotic Assay Medium (AAM), Dextrose Tryptone Agar (DTA) and Tryptic Soy Agar (TSA) with Ca^{2+ added to a final concentration of 100 p.p.m. were used as recovery media. No significant differences were seen between D-values obtained except in the case of strain 12980 when comparing TSA with the other media and for strain 7953 comparing AAM and DTA. The optimum incubation temperature was slightly lower for heated than for unheated spores of} each strain, although, in general, 50 °C was adequate. Higher D-values were obtained at 50–55 °C. The effects of the pH of the medium in the range 5.0–7.0 and the addition of starch and phosphate on heat resistance have also been investigated. Maximum colony counts of heated spores were obtained at pH 7.0 and decreased as pH fell. D-values were significantly lower at pH ≤ 5.5. Increasing the concentration of phosphate in the recovery medium from 0 to 0.2% resulted in a progressive decrease in spore recovery and D-values. The addition of starch improved recoverability. The z-values obtained for the four strains studied under the different recovery conditions were similar with a mean value of 7.58 °C ± 0.28.     

Understanding and Planning for Organized Community Sport in Public Parks: A Case Study of Policy and Practice in Perth

Emerging shortages of community sporting facilities in Australian cities have led to calls for increased provision of playing fields through public park and open space planning processes. Drawing on a broad literature review and a specific case study of metropolitan Perth, this paper evaluates the value of organised sport as a function of contemporary public parks before proposing policy and practice to ensure that such an increase complements broader urban planning agendas. An analysis of the evolution of public park planning shows that, while the provision of organised sport was the primary function of public parks in the middle of the twentieth century, contemporary park planning is characterised by the embrace of broader ecological planning concepts such as green infrastructure and ecosystem services. These broader concepts are then applied to understand the value of organised sport held within public parks, including its unique benefits and notable limitations when considered alongside other forms of outdoor recreation. With these limitations in mind, this paper concludes by identifying existing and future policy and practice that can help to ensure that demand for community sporting facilities in new residential areas can be met in a manner that complements this ecological role for local parks.     

Baseline Stiffness Modulates the Non-Linear Response to Stretch of the Extracellular Matrix in Pulmonary Fibrosis

Pulmonary fibrosis (PF) is a progressive disease that disrupts the mechanical homeostasis of the lung extracellular matrix (ECM). These effects are particularly relevant in the lung context, given the dynamic nature of cyclic stretch that the ECM is continuously subjected to during breathing. This work uses an in vivo model of pulmonary fibrosis to characterize the macro- and micromechanical properties of lung ECM subjected to stretch. To that aim, we have compared the micromechanical properties of fibrotic ECM in baseline and under stretch conditions, using a novel combination of Atomic Force Microscopy (AFM) and a stretchable membrane-based chip. At the macroscale, fibrotic ECM displayed strain-hardening, with a stiffness one order of magnitude higher than its healthy counterpart. Conversely, at the microscale, we found a switch in the stretch-induced mechanical behaviour of the lung ECM from strain-hardening at physiological ECM stiffnesses to strain-softening at fibrotic ECM stiffnesses. Similarly, we observed solidification of healthy ECM versus fluidization of fibrotic ECM in response to stretch. Our results suggest that the mechanical behaviour of fibrotic ECM under stretch involves a potential built-in mechanotransduction mechanism that may slow down the progression of PF by steering resident fibroblasts away from a pro-fibrotic profile.     

A gas flow method for determination of in‐plane permeability of fiber preforms

Resin flow plays a crucial role in many composite manufacturing processes. The most important parameters used in modeling and designing mold filling are the permeability of the fibrous preform, which is a kind of flow conductance and a property of the reinforcement, and the viscosity of the resin. The extent reaction, or degree of cure, is also important and causes change of chemical during mold filling. To determine the permeability of fiber preform searchers have been using liquid flow analysis. In this study, a new scheme for determining permeability using gas flow is proposed. In conventional liquid flow methods, radial propagation of the polymer into a porous medium is measured and used to determine permeability, whereas in the gas flow method, the several different preform geometries is measured and used. The effectiveness of the gas flow method was verified by comparing it with conventional methods.     

Numerical and spectral investigations of Trefftz infinite elements

The numerical and spectral performance of novel infinite elements for exterior problems of time‐harmonic acoustics are examined. The formulation is based on a functional which provides a general framework for domain‐based computation of exterior problems. Two prominent features simplify the task of discretization: the infinite elements mesh the interface only and need not match the finite elements on the interface. Various infinite element approximations for two‐dimensional configurations with circular interfaces are reviewed. Numerical results demonstrate the good performance of these schemes. A simple study points to the proper interpretation of spectral results for the formulation. The spectral properties of these infinite elements are examined with a view to the representation of physics and efficient numerical solution. Copyright © 1999 John Wiley & Sons, Ltd.     

A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies

Polypropylene (PP) mesh is well-known as a gold standard of all prosthetic materials of choice for the reinforcement of soft tissues in case of hernia, organ prolapse, and urinary incontinence. The adverse effects that follow surgical mesh implantation remain an unmet medical challenge. Herein, it is outlined a new approach to allow viability and adhesion of human menstrual blood-derived mesenchymal stromal cells (MenSCs) on PP surgical meshes. A multilayered fibrin coating, based on fibrinogen and thrombin from a commercial fibrin sealant, was optimized to guarantee a homogeneous and stratified film on PP mesh. MenSCs were seeded on the optimized fibrin-coated meshes and their adhesion, viability, phenotype, gene expression, and immunomodulatory capacity were fully evaluated. This coating guaranteed MenSC viability, adhesion and did not trigger any change in their stemness and inflammatory profile. Additionally, MenSCs seeded on fibrin-coated meshes significantly decreased CD4+ and CD8+ T cell proliferation, compared to in vitro stimulated lymphocytes (p < 0.0001). Hence, the proposed fibrin coating for PP surgical meshes may allow the local administration of stromal cells and the reduction of the exacerbated inflammatory response following mesh implantation surgery. Reproducible and easy to adapt to other cell types, this method undoubtedly requires a multidisciplinary and translational approach to be improved for future clinical uses.